Abstract Pulsed Electric field (PEF) cell lysis technology, as a new technology, has been widely used in the fields of food sterilization. Nowadays, there are still several problems in PEF cell lysis: low cell lysis rate, high temperature rise, the presence of low velocity regions, etc. In this study, COMSOL Multiphysics was used to conduct simulation research, and the electric field, flow field and temperature field of plate treatment chamber, coaxial treatment chamber and co-field treatment chamber were coupled to simulate, and the advantages and disadvantages of the three classical treatment chamber structures were analyzed. In addition, this paper proposes a new structure of the co-field treatment chamber. Through the comparison and simulation of the coupling between our design and other four structures, the electric field intensity of our design treatment area is 20.03 kV/m, which is 46.2% higher than the highest of others. The temperature rise within 1s is 0.93 K, which is 2/3 lower than others. Our design almost eliminates the peak electric field area at the junction between the inside of the electrode and the insulator, and the maximum electric field intensity in this area is reduced by 24.4% compared with others.

Effects of various factors, such as pH, ionic strength, glutaraldehyde concentration, enzyme amount and immobilization time, on enzyme activity were investigated. The immobilization conditions were optimized by orthogonal experiments. Characterizations of immobilized nuclease p1 were also evaluated. Through orthogonal optimization, the optimal immobilization conditions were as follows: pH 5.6, ionic strength 0.125, glutaraldehyde concentration 0.20% and immobilization time 2.0 h. Optimal pH of immobilized enzyme was 5.8. Optimal temperature of immobilized enzyme was 70ºC. Thermal, operational and storage stabilities of the enzyme were improved after it was immobilized on DEAE cellulose. Michaelis constant Km of immobilized enzyme at 69ºC was found to be 27.21 g/l by the Lineweaver-Burk plot.